MeDIP-sequencing combines methyl-DNA immunoprecipitation (MeDIP) with next-generation sequencing technology to create moderate resolution methylation profiles of DNA methylation state on a genome-wide scale. KangChen offers integrated MeDIP-sequencing service from sequencing library preparation to comprehensive data analysis.
Highlights
Genome-Wide Analysis: Whole-genome analysis of DNA methylation patterns, not limited to array features or candidate sequences
High Signal-to-Noise Ratio: Lower background than MeDIP-chip, no cross hybridization
High Resolution: Positional precision of mapped binding sites ±50bp
Wide Dynamic Range: Robust quantification of the methylation state by simply counting the frequency of reads that map to the same region
Low Sample Amount Required: 10ng fragmented, MeDIP enriched DNA
MeDIP-Sequencing Service
1. Sequencing Library Preparation
Obtain methylated DNA fragments by MeDIP
Ligate Adapters to DNA fragments
Size select the library
Enrich the adapter-modified DNA fragments by PCR
2. Cluster Generation
Attach DNA to surface of the flow cell
Bridge PCR amplification
3. Sequencing
Determine the sequencing cycle
Sequence
Image processing and base calling
4. Data analysis
Raw data generating
Genomic mapping and annotation
DNA methylation state analysis (peak finding)
Custom data analysis
Data report
Services