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 mRNA Sequencing Service
mRNA sequencing allows profiling the whole population of mRNA in any eukaryotic species and enables mapping and digital quantification of all the transcripts. It also provides information of alternative splice site, novel transcripts, unknown isoform, rare transcripts, and cSNPs. KangChen offers integrated mRNA Sequencing service from sequencing library preparation to comprehensive data analysis.
 
Highlights
Digital Readout: Count the numerical frequency of each sequence in your library population and effectively eliminate background noise
Universal Discovery: Detect mRNA transcripts in any eukaryotic organism with no need of prior identification or annotation
Tunable Coverage: Digital sequencing data are convenient for researchers to tune coverage depth with specific design and discover rare transcript with low read
High-Quality Results: High sensitivity and accuracy performance allows confident quantification of differential expression between samples
 
mRNA Sequencing Service
1. Sequencing Library Preparation
     Purify mRNA from total RNA
     Fragment the mRNA
     Synthesize cDNA
     Ligate adapters to DNA framents
     Purify cDNA templates and enrich the purified cDNA templates by PCR
2. Cluster Generation
     Attach DNA to surface of the flow cell
     Bridge PCR amplification
3. Sequencing
     Determine the sequencing cycle
     Sequence
     Image processing and base calling
4. Data Analysis
     Raw data generating
     Genomic mapping and annotation
     Differential gene expression analysis
     Custom data analysis
     Data report

   


Services
Whole Genome Expression
Real-time PCR Array
mRNA Tag Profiling Service
mRNA Sequencing Service
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