ChIP-sequencing combines Chromatin Immunoprecipitation (ChIP) with next generation sequencing technology to identify and quantify the binding sites of any DNA-associated proteins (such as transcriptional factors, RNA polymerase, histones) on a genome-wide scale with no constrains. KangChen offers integrated ChIP-sequencing service from sequencing library preparation to comprehensive data analysis.
Highlights
HighQualityData: Positional precision of mapped binding sites ±50bp
WideDynamicRange: Robust quantification for determining binding specificities of varying strengths
HighSignal-to-NoiseRatio: Lower background than ChIP-chip, no cross hybridization
Genome-WideAnalysis: Identify any binding sites in promoters, enhancers, exons, introns, even in repetitive regions, not limited to array features or candidate sequences
Low Starting Material Requirement: Robust output from as little as 10ng of precious input
ChIP-Sequencing Service
1. Sequencing Library Preparation
Obtain target DNA fragments by ChIP
Ligat Adapters to DNA fragments
Size select the library
Enrich the adapter-modified DNA framents by PCR
2. Cluster Generation
Attach DNA to surface of the flow cell
Bridge PCR amplification
3. Sequencing
Determine the sequencing cycle
Sequence
Image processing and base calling
4. Data Analysis
Raw data generating
Genomic mapping and annotation
Enriched region identification (peak finding)
Custom data analysis
Data report
Services