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 mRNA Tag Profiling Service
mRNA tag profiling is a revolutionary approach to expression analysis. It can profile any polyadenylated mRNA from any organism, detect rare transcripts, and validate microarray results with quantification comparable to qPCR. KangChen offers integrated mRNA tag profiling service from sequencing library preparation to comprehensive data analysis.
 
Highlights
No sequence knowledge required: Universal platform to study any transcript
Tunable Coverage: Almost unlimited dynamic range for rare transcript discovery
High Sensitivity: Four million tags per sample yields an average of 12 counts for transcripts at one copy per cell
Orthogonal validation: Genome-wide data comparable to qPCR validation of microarrays
 
microRNA Tag Sequencing Service
1. Sequencing Library Preparation
    Isolate poly-A mRNA
    Synthesize cDNA and restriction digest with DpnII or NlaIII
    Ligate GEX DpnII or NlaIII adapter1
    Restriction digest with MmeI
    Ligate GEX adapter 2
    Enrich the adapter-ligated cDNA construct using PCR
    Purify amplified cDNA Construct
2. Cluster Generation
    Attach DNA to surface of the flow cell
    Bridge PCR amplification
3. Sequencing
    Determine the sequencing cycle
    Sequence
    Image processing and base calling
4. Data Analysis
    Raw data generating
    Genome mapping and annotation
    Differential gene expression analysis
    Custom data analysis
    Data report
 

Services
Whole Genome Expression
Real-time PCR Array
mRNA Tag Profiling Service
mRNA Sequencing Service
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