mRNA tag profiling is a revolutionary approach to expression analysis. It can profile any polyadenylated mRNA from any organism, detect rare transcripts, and validate microarray results with quantification comparable to qPCR. KangChen offers integrated mRNA tag profiling service from sequencing library preparation to comprehensive data analysis.
Highlights
No sequence knowledge required: Universal platform to study any transcript
Tunable Coverage: Almost unlimited dynamic range for rare transcript discovery
High Sensitivity: Four million tags per sample yields an average of 12 counts for transcripts at one copy per cell
Orthogonal validation: Genome-wide data comparable to qPCR validation of microarrays
microRNA Tag Sequencing Service
1. Sequencing Library Preparation
Isolate poly-A mRNA
Synthesize cDNA and restriction digest with DpnII or NlaIII
Ligate GEX DpnII or NlaIII adapter1
Restriction digest with MmeI
Ligate GEX adapter 2
Enrich the adapter-ligated cDNA construct using PCR
Purify amplified cDNA Construct
2. Cluster Generation
Attach DNA to surface of the flow cell
Bridge PCR amplification
3. Sequencing
Determine the sequencing cycle
Sequence
Image processing and base calling
4. Data Analysis
Raw data generating
Genome mapping and annotation
Differential gene expression analysis
Custom data analysis
Data report
Services