SABiosciences is a leading provider of pathway-focused microarrays. Its PCR Arrays, combined microarray profiling capabilities with real-time PCR performance, are the most reliable tools for analyzing the expression of a focused panel of genes.

 

SABiosciences designed and launched over 70 Oligo PCR Arrays (including human, mouse and rat), which contain the most up-to-date and popular pathways, such as Apoptosis, Cell Cycle, Cancer, common diseases, Common Cytokines, Extracellular Matrix and Adhesion Molecules, Neurogenesis and Neural Stem Cell, Signal Transduction in Cancer, Stem Cell, Toxicology & Drug Resistance.

 

1.   RNA Extraction

2.   Determine the purity and concentration of total RNA with Nanodrop ND1000

       Determine the integrity of total RNA with Agilent’s 2100 BioAnalyzer or Denaturing Gel Electrophoresis

3.   cDNA synthesis

4.   Realtime PCR

5.   Data extraction and preliminary analysis

6.   Complete report including:

             3D profile

            

             Scatter plot

            

             Amplification curve

            

             Melting curve