KangChen Bio-tech provides complete ChIP-on-chip service from sample to results. This service, which is available to academic and commercial researchers, employs Agilent’s and NimbleGen’s complete microarray platform.
ChIP-on-chip service provided by Kangchen Biotech:
1. Cross link proteins to DNA using formaldehyde
2. Sonication to shear chromatin DNA
3. ChIP and Purification
4. Amplification using Sigma WGA kit and Purification
5. Labeling using Cy3/Cy5
6. Hybridization
7. Scan, data extraction and preliminary analysis
8. Complete report including:
Scanned array image:
Partial enlarged array image:
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Raw data
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log2-ratio data
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Scatter plot, MA-plot

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Peaks GFF file
Agilent ChIP-chip:
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Agilent’s 60-mer oligonucleotide microarrays (frequently updated content, sequence and annotation information available through Agilent)
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Microarrays manufactured using computer driven inkjet printing process with SurePrint technology
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Well-tested protocols (direct labeling or single round amplification labeling)
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Scanning and feature extraction with Agilent’s DNA Microarray Scanner and SureScan technology
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Data analysis with GeneSpring
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Easily access to probe sequence and annotation information
Agilent |
Slides format |
Resolution |
Description |
Human Promoter |
244K x 2 |
Coverage: -5.5k to +2.5k region
~25 probes/gene
Spacing ~195bp |
UCSC hg17, NCBI Build 35
~17,000 best defined human transcripts |
Mouse Promoter |
244K x 2 |
Coverage: -5.5k to +2.5k region
~25 probes/gene
Spacing ~200bp |
UCSC mm7, NCBI Build 35
~17,000 best defined mouse transcripts |
Human Proximal promoter |
4×44K |
Coverage: -1k to +0.3k region
~3 probes/gene
Spacing ~500bp |
UCSC hg17, NCBI Build35
~17,000 best defined human transcripts |
NimbleGen ChIP-chip
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NimbleGen’s 50-75-mer oligonucleotide probes to equalize the melting temperature
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Microarrays manufactured using photo-mediated synthesis chemistry with its MAS system
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Feature extraction with NimbleGen’s NimbleScan™ software
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Graphical representation of your data with NimbleGen’s SignalMap™ data browser
Catalog Number |
Design Name |
Promoter Upstream Tiling (bp) |
Promoter downstream Tiling(bp) |
C4226-00-01 |
HG18 RefSeq promoter |
2200 |
500 |
C4222-00-01 |
MM8 RefSeq promoter |
2000 |
500 |
C4495001-00-01 |
RN34 RefSeq promoter |
2250 |
500 |