康成生物--Services

ChIP-on-chip is a technology that utilizes microarrays to analyse how regulatory proteins interact with the genome and provides insight into the mechanisms of histone modification and regulation of transcription factor.

KangChen Bio-tech provides complete ChIP-on-chip service from sample to results. This service, which is available to academic and commercial researchers, employs Agilent’s and NimbleGen’s complete microarray platform.

ChIP-on-chip service provided by Kangchen Biotech：

1. Cross link proteins to DNA using formaldehyde

2. Sonication to shear chromatin DNA

3. ChIP and Purification

4. Amplification using Sigma WGA kit and Purification

5. Labeling using Cy3/Cy5

6. Hybridization

7. Scan, data extraction and preliminary analysis

8. Complete report including:

Scanned image

Scanned array image:

Partial enlarged array image:

Raw data
log2-ratio data
Scatter plot, MA-plot
Peaks GFF file

Agilent ChIP-chip:

Agilent’s 60-mer oligonucleotide microarrays (frequently updated content, sequence and annotation information available through Agilent)
Microarrays manufactured using computer driven inkjet printing process with SurePrint technology
Well-tested protocols (direct labeling or single round amplification labeling)
Scanning and feature extraction with Agilent’s DNA Microarray Scanner and SureScan technology
Data analysis with GeneSpring
Easily access to probe sequence and annotation information

Agilent	Slides format	Resolution	Description
Human Promoter	244K x 2	Coverage: -5.5k to +2.5k region ~25 probes/gene Spacing ~195bp	UCSC hg17, NCBI Build 35 ~17,000 best defined human transcripts
Mouse Promoter	244K x 2	Coverage: -5.5k to +2.5k region ~25 probes/gene Spacing ~200bp	UCSC mm7, NCBI Build 35 ~17,000 best defined mouse transcripts
Human Proximal promoter	4×44K	Coverage: -1k to +0.3k region ~3 probes/gene Spacing ~500bp	UCSC hg17, NCBI Build35 ~17,000 best defined human transcripts

NimbleGen ChIP-chip

NimbleGen’s 50-75-mer oligonucleotide probes to equalize the melting temperature
Microarrays manufactured using photo-mediated synthesis chemistry with its MAS system
Feature extraction with NimbleGen’s NimbleScan™ software
Graphical representation of your data with NimbleGen’s SignalMap™ data browser

Catalog Number	Design Name	Promoter Upstream Tiling (bp)	Promoter downstream Tiling(bp)
C4226-00-01	HG18 RefSeq promoter	2200	500
C4222-00-01	MM8 RefSeq promoter	2000	500
C4495001-00-01	RN34 RefSeq promoter	2250	500