microRNA sequecing based on the next generation sequencing technology allows us to identify and profile all microRNAs from any organism without prior sequence or secondary structure information. KangChen offers integrated microRNA sequencing service from sequencing library preparation to comprehensive data analysis.
Highlights
Superior data: Robust microRNA sequencing and quantification
Universal Discovery: No prior sequence or secondary structure information required
Wide Dynamic Range: Up to four million reads of microRNA can be generated in each flow cell channel
Customizable Size Selection: Any microRNA between 20-25 nucleotides can be investigated
microRNA Sequencing Service
1. Sequencing Library Preparation
Isolate microRNA
Ligate 5' RNA adapter and 3' RNA adapter
Reverse transcribe and amplify the microRNA ligated with adapters
Purify the amplified cDNA construct
2. Cluster Generation
Attach DNA to surface of the flow cell
Bridge PCR amplification
3. Sequencing
Determince the sequencing cycle
Sequence
Image processing and base calling
4. Data Analysis
Raw data generating
Genomic mapping and annotation
Differential gene expression analysis
Custom data analysis
Data report
Services